Protein Method Blocking Buffers
Bioworld Horse Blocker, 200 mL
Horse Blocker Blot blocking buffer used to block non-specific binding site for antibodies on the membrane. Technical Information: 10% Horse serum, 0.02% sodium azide in PBS
Bioworld BBS-Casein (10X), 500 mL
BSS-Casein, supplied in 10X PBS and 5% Csein. Technical Information:10X Concentrate has a working pH of 8.5 ( +/- 0.1)Formulated in a borate buffered physiologic saline bufferSold as 2 x 100mL or 500mL.
Bioworld NAP- (Non-Animal Protein) Blocker, 1 L
NAP (Non Animal Protein)-BLOCKER provides improved assay sensitivity, clean background and high signal-to-background ratio. Produced from non-animal proteins that are free of biotin and other agents that interfere with immunoassays. Will not cross react with animal source antigens or primary and secondary antibodies. Supplied as a 4X solution, simply mix with water to obtain a 1X ready to use buffer for nitrocellulose and PVDF membranes. Suitable for up to 100 mini-gel blots using plastic bags for reduced volume. Usually Ships within 24 HoursAdvantage:Reduces risk of non-specific binding Lowers background Improves assay sensitivityApplication: Western blot, dot blot & ELISA experiments
Biotang Inc BLOTTO Antifoam In PBS (with azide), 250 ml
BLOTTO Antifoam In PBS (with azide), 250 ml.
Biotang Inc BLOTTO/TWEEN 20 IN TBS 250 ML, IBB-161. 5% nonfat dry milk, 0.05% Tween-20 in TBS (50 mM Tris-HCl and 150 mM NaCl, pH 7.4 +/- 0.2). Prepared in 18.2 megohms water and filtered through 1 micron filter.
BLOTTO/TWEEN 20 IN TBS 250 ML, IBB-161. 5% nonfat dry milk, 0.05% Tween-20 in TBS (50 mM Tris-HCl and 150 mM NaCl, pH 7.4 +/- 0.2). Prepared in 18.2 megohms water and filtered through 1 micron filter.
Biotang Inc BSA- Blocking Buffer in TBS and Tween-20, 250 ml
BSA- Blocking Buffer in TBS and Tween-20, 250 ml.
Biotang Inc GELATIN- Blocking Buffer in PBS (with azide), 500 ml
GELATIN- Blocking Buffer in PBS (with azide), 500 ml.
Biotang Inc BLOTTO TWEEN IN PBS 250 ML, IBB-151, 5% nonfat dry milk with 0.05% Tween-20 in PBS (10 mM Sodium phosphate, 2 mM potassium phosphate,10 mM KCl,137 mM NaCl), 7.4 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
BLOTTO TWEEN IN PBS 250 ML, IBB-151, 5% nonfat dry milk with 0.05% Tween-20 in PBS (10 mM Sodium phosphate, 2 mM potassium phosphate,10 mM KCl,137 mM NaCl), 7.4 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
Biotang Inc BLOTTO/Tween 20 in TBS (with azide), 250 ml
BLOTTO/Tween 20 in TBS (with azide), 250 ml.
Chondrex Inc ChonBlock Detection Antibody Dilution Buffer, 100ml
100 mL of a mammalian protein-based ELISA buffer for dilution of detection/secondary antibody solutions. Please visit www.chondrex.com or email Chondrex, Inc. at support@chondrex.com for more information on how ChonBlock can improve your ELISA results. pH 7.8. Contains 0.4% Tween-20 and 0.04% ProClin 300.
LAB BIOREAGENTS StrongBlock III Buffer 250ml
Lab Bioreagents StrongBlocks (I, II, III) are newly developed blocking buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays The blocking buffer is to block excess binding sites on the micro-plate for reducing non-specific binding signal, by which the assay sensitivity and specificity are improved. As many factors can influence nonspecific binding, there is no single blocking buffer that is ideal for each assay. For example, depending on the recognition of detection probes from different assays, one individual blocking buffer may cross-react with detection probe. StrongBlocks (I, II, III) are a variety of buffers designed by different blocker components. During the assay, the test different blocking buffers helps to develop a immunoassay with low background noise and high signal-to-noise.
Cell Signaling Technology Phosphate Buffered Saline with Tween 20 (PBST-20X) 1000 ml
Phosphate Buffered Saline with Tween 20 (PBST-20X) 1000 ml
Bioworld Denhardt's Solution (100X), 50 mL
Denhardt's Solution, 100X Concentrate Acts as a blocking reagent in hybridizations to help prevent probe binding to open membrane space and non-target nucleic acids. Specifications:Ultra Pure GradePurity: 99.0%pH @ 25°C: 5.0-7.0 Conductivity @ 25°C (µmhos): <350 Abs @ 278nm (5% water): 0.600-0.750 DNAse: None detectedRNAse: None detectedStorage: -15 to -22°C
Amsbio LLC ChonBlock™ Blocking/Sample Dilution ELISA Buffer- 100 ml
ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml
Seracare Life Sciences Inc 10X PBS W/ TWEEN 20(PBST)200ML
10X Phosphate-buffered Saline with Tween 20 (PBST) is ideal for use as a wash buffer in general immunoassay procedures. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. pH=7.4. Conductivity controlled.